TNFa-producing Macrophages Determine Subtype Identity and Prognosis via AP1 Enhancer Reprogramming in Pancreatic Cancer [TNFa-treatment RNA-seq]

In pancreatic cancer, classical and basal-like subtypes are identified as two major PDAC subtypes. Our previous data showed that PDAC tumor cells can switch between the two phenotypes through transcriptional reprogramming particularly in response to inflammatory cues. In this study, we were trying to understand TNFa mediated transcription regulatory network in pancreatic cancer. Hence, we performed RNA-seq in TNFa treated classical cells as well as aqua dest control. Overall design: RNA-seq was performed in aqua dest treated CAPAN1 control samples, as well as 18 h TNFa treated CAPAN1 cells (10 ng/ul). Three biological repeats were used in each group.

在胰腺癌中，经典型（classical）与基底样型（basal-like）是胰腺导管腺癌（PDAC, Pancreatic Ductal Adenocarcinoma）的两大主要亚型。我们此前的研究数据显示，胰腺导管腺癌细胞可通过转录重编程在两种表型间相互转换，该过程尤其响应炎症信号刺激。本研究旨在解析肿瘤坏死因子α（TNFα, Tumor Necrosis Factor α）介导的胰腺癌转录调控网络。为此，我们对经肿瘤坏死因子α处理的经典型胰腺腺癌细胞以及灭菌蒸馏水（aqua dest.）对照组细胞开展了RNA测序（RNA-seq）实验。实验整体设计：分别对经灭菌蒸馏水处理的CAPAN1对照组细胞，以及经10 ng/μl肿瘤坏死因子α处理18小时的CAPAN1细胞进行RNA测序，每组均设置3次生物学重复。