Whole genome MinION sequencing of derivatives of E.coli 83972

We have created fluorescent derivatives of the E.coli strain 83972, constitutively expressing either red fluorescence (mKate protein) or green fluorescence (gfp protein) from the chromosome. In addition, we have selected rifampicin and ciprofloxacin resistant versions of these strains. Using Oxford Nanopore MinION sequencing we sequenced the whole genomes of these strains (as well as the original 83972 strain).

我们构建了大肠杆菌（E. coli）菌株83972的荧光衍生株，该类菌株可从染色体上组成型表达红色荧光（mKate蛋白）或绿色荧光（gfp蛋白）。此外，我们还筛选得到了携带利福平（rifampicin）和环丙沙星（ciprofloxacin）耐药性的上述衍生株。我们采用牛津纳米孔MinION测序平台，对上述菌株及原始83972菌株开展了全基因组测序。