RNA-seq analysis of Hypoxia and Normoxia cultured cancer cells

Purpose: To study differential mRNA and precursor miRNA expression under hypoxia exposure in cancer cells. We treated ovarian cancer cell line A2780 under hypoxia condition at different time points. Normoxi acultured cells at corresponding time point were used as controls. Overall design: A2780 cancer cells, cultured under hypoxia and normoxia. Time points tested: 6hr, 48hr, 6days.

研究目的：探究癌细胞经低氧暴露后的差异信使RNA（mRNA）与前体微小RNA（precursor miRNA）表达谱。本研究对卵巢癌细胞系A2780施加不同时长的低氧处理，以对应时间点常氧培养的细胞作为对照。
整体实验设计：以卵巢癌细胞系A2780为受试对象，分别在低氧与常氧环境下培养，设置的检测时间点为6小时、48小时及6天。