Transcriptome sequencing of Cleome hassleriana for different tissues

We have sequenced the whole genome of Cleome hassleriana by NGS [SRA accession number SRA058749] and build a reference sequence for this genome. In order to improve quality of gene models, a mix transcriptome sample extracted from the bud, leaf, petiole, stems and flowers of Cleome. Overall design: A mixed RNA pool extracted from the tissues of bud, leaf, petiole, stems and flower is sequenced for Cleome hassleriana.

本研究采用下一代测序技术（Next Generation Sequencing, NGS）对醉蝶花（Cleome hassleriana）的全基因组进行测序，相关测序数据的序列读取存档（Sequence Read Archive, SRA）登录号为SRA058749，并构建了该物种的基因组参考序列。为提升基因模型的质量，我们制备了混合转录组样本，该样本提取自醉蝶花的芽、叶、叶柄、茎及花组织。整体实验设计为：将从醉蝶花的芽、叶、叶柄、茎及花组织中提取的RNA混合为RNA池，对该混合转录组样本进行测序。