miR101 overexpression vs. EZH2 RNAi. Homo sapiens

Transcriptional analysis of genes in SKBr3 cells. Enhancer of zeste homolog 2 (EZH2) is a mammalian histone methyltransferase that contributes to the epigenetic silencing of target genes and regulates the survival and metastasis of cancer cells. EZH2 is overexpressed in aggressive solid tumors by mechanisms that remain unclear. Here we show that the expression and function of EZH2 in cancer cell lines are inhibited by microRNA-101 (miR-101). Overall design: Two-condition experiment: miR101 overexpression vs. EZH2 RNAi in SKBr3 breast cancer cells.

SKBr3细胞的基因转录组分析。zeste同源物2增强子（Enhancer of zeste homolog 2，EZH2）是一类哺乳动物组蛋白甲基转移酶，可介导靶基因的表观遗传沉默，并调控癌细胞的存活与转移。侵袭性实体瘤中存在EZH2过表达现象，但其具体调控机制尚未明确。本研究证实，microRNA-101（miR-101）可抑制癌细胞系中EZH2的表达与功能。实验设计：采用双处理组实验方案，在SKBr3乳腺癌细胞中分别进行miR-101过表达与EZH2 RNA干扰（RNAi）处理。