Additional file 2: of Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

Collection of all off-targets and their frequencies from all off-target studies. The fields of the table are: name of the guide (guide), its sequence (guideSeq), the sequence of the off-target (otSeq), the specificity score of the guide determined with up to four mismatches (guideSpecScore4MM), the GC content of the guide (guideGcCont), the fraction of reads or lentiviral integration sites for this off-target (readFraction), the number of mismatches of the off-target to the guide (mismatches), the Hsu et al. off-target score (outscore), a string indicating with stars where the mismatches are located over the length of the guide sequence (otLogo), the minimal number of mismatches if a deletion is allowed in the guide sequence (bulgeRnaMmCount), the guide sequence with this deletion marked in parentheses (bulgeRnaGuideSeq), the minimal number of mismatches if a deletion is allowed in the off-target sequence (bulgeDnaMmCount), and the off-target sequence with the deletion marked (bulgeDnaOtSeq). Bulge information is shown only if the number of mismatches obtained by the deletion is at least three less than the number of mismatches without the deletion; otherwise the fields are set to “−1”. (TSV 98 kb)

本数据集收录了所有脱靶效应研究中的全部脱靶位点及其出现频率。表格包含以下字段：向导序列名称（guide）、向导序列（guideSeq）、脱靶位点序列（otSeq）、最多4个错配条件下向导序列的特异性评分（guideSpecScore4MM）、向导序列的GC含量（guideGcCont）、该脱靶位点对应的测序读段或慢病毒整合位点占比（readFraction）、脱靶位点与向导序列的错配碱基数（mismatches）、Hsu等人提出的脱靶效应评分（outscore）、以星号标记错配在向导序列全长上位置的字符串（otLogo）、允许向导序列存在缺失时的最小错配碱基数（bulgeRnaMmCount）、带括号标记缺失位置的向导序列（bulgeRnaGuideSeq）、允许脱靶序列存在缺失时的最小错配碱基数（bulgeDnaMmCount）、带括号标记缺失位置的脱靶位点序列（bulgeDnaOtSeq）。仅当通过序列缺失计算得到的错配碱基数比无缺失时少至少3个时，才会展示凸起（bulge）相关信息；否则上述字段将被设为"−1"。（TSV 格式，文件大小98 KB）