CIL:41367

The image shows a portion of a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows the plasma membrane with cytoplasm above and medium below. Numerous vesicles are present in the cytoplasm. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.

本图像展示了基于X射线显微镜（X-ray microscopy）构建的小鼠腺癌细胞三维断层重建结果的切片片段。研究人员通过在液态乙烷中实施快速冷冻（plunge freezing）对活细胞进行冷冻固定，并采用X射线显微镜对未染色的玻璃化样品进行观测，该显微镜所用波长可在水与有机物质间形成高对比度。图像采集过程中，样品倾斜角度覆盖-60°至+60°的范围，采集间隔为1°。本图像为计算机断层扫描图（computed tomogram）在x-y平面上的9.8 nm厚切片，可见质膜（plasma membrane）上方为细胞质，下方为培养基，细胞质内分布有大量囊泡（vesicles）。本图像的分辨率约为36 nm（瑞利判据（Rayleigh Criterion））与70 nm（傅里叶环相关（Fourier Ring Correlation）），后者对应约35 nm的半间距分辨率。本CIL组还收录了该研究中的其他X射线图像。另见：W G Muller等，2012年，《基于软X射线断层成像构建哺乳动物细胞超微结构图谱》，《结构生物学杂志》（J Struct Biol）177:179-192。