Ticks, and tick-borne bacterial pathogens found on hard ticks (Acari: Ixodidae) on cattle in the Central River Region of The Gambia

Ticks are significant vectors of pathogens affecting both animals and humans, with the climate in Sub-Saharan Africa providing ideal conditions for their growth. However, there is limited data on ticks and tick-borne pathogens (T&TBPs) in cattle in The Gambia. This study aimed to identify tick species on cattle and conduct molecular screening for T&TBPs. A total of 92 ticks were collected from 306 indigenous cattle. Ticks were first identified morphologically using taxonomic keys and then confirmed molecularly through DNA sequencing. DNA was extracted from the right fourth leg of six representative ticks for species confirmation, while 77 whole adult ticks were used for screening T&TBPs. Screening PCR assays targeted Anaplasma marginale msp1β gene, Ehrlichia spp. dsb gene, and hemotropic Mycoplasma spp. 16S rRNA gene. Ehrlichia-positive samples underwent additional assays targeting the sodB, 16S rRNA, and groEL genes, followed by Sanger sequencing and phylogenetic analy..., , , # Ticks, and tick-borne bacterial pathogens found on hard ticks (Acari: Ixodidae) on cattle in the Central River Region of The Gambia

Access this dataset on Dryad: DOI: 10.5061/dryad.34tmpg4v6

## Description:

Our data contains one file:

Data\_Ticks\_from\_Gambia.xlsx

Data Structure and Potential Use: This dataset is organized in an Excel spreadsheet format with separate sheets and clearly labeled columns to facilitate ease of use.

### Sheet 1: Identified ticks

Tick Metadata Sample ID: Unique identifier for each tick.

Host Animal ID: Identifier linking the tick to the individual cattle host.

Tick Species: Determined using morphological keys (e.g., *Hyalomma marginatum*, *Amblyomma variegatum*, etc.).

Sex & Life Stage: Recorded as male (M), female (F), or nymph.

### Sheet 2: TBPs analysed

PCR Results Sample ID: Cross-referenced with Sheet 1.

Pathogen Target Gene: Indicates which gene was tested (e.g., dsb, 16S rRNA, sodB for Ehrlichia spp.).

PCR Result: Positive or negative....,

蜱虫（Ticks）是一类可传播病原体的重要媒介，可同时感染动物与人类，撒哈拉以南非洲的气候为其种群繁衍提供了理想条件。但目前冈比亚境内牛群相关的蜱虫与蜱传病原体（Ticks and Tick-borne Pathogens，简称T&TBPs）相关数据仍较为匮乏。本研究旨在鉴定牛体表的蜱虫种类，并对T&TBPs开展分子筛查。本研究从306头本地土种牛身上共采集到92只蜱虫。研究人员首先通过分类学检索表对蜱虫进行形态学鉴定，随后通过DNA测序完成分子学验证。研究人员从6只代表性蜱虫的右侧第四足提取DNA以完成种类确认，同时选取77只完整成虫蜱虫用于T&TBPs筛查。本次筛查采用的聚合酶链式反应（PCR）靶向检测边缘无浆体（Anaplasma marginale）的msp1β基因、埃立克体属（Ehrlichia spp.）的dsb基因以及嗜血支原体属（hemotropic Mycoplasma spp.）的16S rRNA基因。埃立克体检测呈阳性的样本将进一步开展针对sodB、16S rRNA及groEL基因的检测，随后进行桑格测序与系统发育分析……# 冈比亚中部河区域牛体表硬蜱（蛛形纲：硬蜱科）携带的蜱虫及蜱传细菌性病原体

可在Dryad平台获取本数据集：DOI: 10.5061/dryad.34tmpg4v6

## 数据集说明：
本数据集包含1个文件：Data_Ticks_from_Gambia.xlsx

### 数据集结构与潜在用途：
本数据集采用Excel电子表格格式组织，包含多个独立工作表，且列标签清晰明确，便于用户使用。

### 工作表1：已鉴定蜱虫
- 蜱虫元数据 样本ID：每只蜱虫的唯一标识符。
- 宿主动物ID：用于关联蜱虫与其宿主牛个体的标识符。
- 蜱虫种类：通过形态分类键（如*边缘璃眼蜱（Hyalomma marginatum）*、*三色花蜱（Amblyomma variegatum）*等）确定。
- 性别与生命阶段：记录为雄性（M）、雌性（F）或若虫。

### 工作表2：已分析的蜱传病原体
- PCR结果 样本ID：与工作表1相互参照。
- 病原体靶基因：标明所检测的基因（如针对埃立克体属（Ehrlichia spp.）的dsb、16S rRNA、sodB基因等）。
- PCR结果：阳性或阴性……