Investigating the impact of GalNAc-T2 overexpression on proteomics and glycoproteomics in lung cell

GalNAc-T2 was identified as a novel antiviral factor that promotes viral clearance and contributes to reduced disease severity.This project aimed to investigate the impact of GalNAc-T2 overexpression on proteomics and glycoproteomics in lung cell. We employ SILAC labeling, lectin-based affinity enrichment, and LC-ETD MS/MS to characterize the regulated proteins and glycosylation sites resulting from GalNAc-T2 overexpression.

GalNAc-T2被鉴定为一种新型抗病毒因子，可促进病毒清除并有助于降低疾病严重程度。本研究旨在探究GalNAc-T2过表达对肺细胞蛋白质组学与糖蛋白质组学的影响。我们采用SILAC（细胞培养氨基酸稳定同位素标记）标记、基于凝集素的亲和富集技术以及LC-ETD MS/MS（液相色谱-电子转移解离串联质谱），对GalNAc-T2过表达所调控的蛋白质及糖基化位点进行表征。