Combining Stem Cell Rejuvenation and Senescence Targeting to Synergistically Extend Lifespan

We combine transient stem cell rejuvenation with targeted removal of senescent cells to test the hypothesis that simultaneously targeting both cell-fate based aging mechanisms will maximize life and health span benefits. Overall design: Drosophila midgut RNA profiles of tubGal80ts > UAS-TdTomato (WT), armGal4; tubGal80ts > UAS-OKSM (OKSM), armGal4; tubGal80ts > UAS-Sen (Sen) and armGal4; tubGal80ts > UAS-Sen; UAS-OKSM (Sen_OKSM); tubGal80ts > UAS-TdTomato (WT), esgGal4; tubGal80ts > UAS-OKSM (OKSM), esgGal4; tubGal80ts > UAS-Sen (Sen) and esgGal4; tubGal80ts > UAS-Sen; UAS-OKSM (Sen_OKSM) Updates: [Sept. 20, 2022] The GSM6058923 and GSM6058926 metadata were swapped in accordance with the sample identities. The associated data files did not change but were renamed accordingly.

本研究将瞬时干细胞年轻化与靶向清除衰老细胞相结合，旨在验证同时靶向两类基于细胞命运的衰老机制可最大化延长寿命与健康寿命的假说。整体实验设计：涵盖8组果蝇中肠转录组谱，具体分组如下：第一组为tubGal80ts > UAS-TdTomato（野生型，Wild Type）、armGal4; tubGal80ts > UAS-OKSM（OKSM组）、armGal4; tubGal80ts > UAS-Sen（Sen组）及armGal4; tubGal80ts > UAS-Sen; UAS-OKSM（Sen_OKSM组）；第二组为tubGal80ts > UAS-TdTomato（野生型，Wild Type）、esgGal4; tubGal80ts > UAS-OKSM（OKSM组）、esgGal4; tubGal80ts > UAS-Sen（Sen组）及esgGal4; tubGal80ts > UAS-Sen; UAS-OKSM（Sen_OKSM组）。更新说明：[2022年9月20日] 依据样本标识，已交换GSM6058923与GSM6058926的元数据。关联数据文件未发生改动，但已同步完成重命名。