A Workflow for Bronchoalveolar Lavage Fluid Sequencing in Guinea Pigs

The aim of this study was to optimise a methodological workflow for 16S rRNA sequencing of bronchoalveolar lavage fluid from guinea pig models with differing microbial statuses. By systematically comparing DNA extraction kits and input concentrations, conditions were identified that improved microbial detection despite substantial host DNA contamination.

本研究旨在优化针对不同微生物定植状态豚鼠模型支气管肺泡灌洗液的16S rRNA测序方法学流程。通过系统比较DNA提取试剂盒与样本起始投入浓度，确定了可在存在大量宿主DNA污染的情况下提升微生物检测效能的实验条件。