Temporal mapping of C/EBPα and –β binding during liver regeneration. Mus musculus

Temporal mapping of C/EBPα and –β binding during liver regeneration reveals dynamic occupancy and specific regulatory codes for homeostatic and cell cycle gene batteries. Overall design: Timeseries ChIP-seq of transcription factors C/EBPα and –β, and Pol II in time points 0, 3, 8, 16, 24, 36, 48, 168 hours after partial hepatectomy. Additionally, an IgG mock to 0h was sequenced, as well as the transcription factor Egr1.

在肝脏再生过程中对C/EBPα与C/EBPβ的结合位点进行时序图谱分析，揭示了稳态基因簇与细胞周期基因簇的动态染色质占据特征及特异性调控密码。实验设计概述：对部分肝切除术后0、3、8、16、24、36、48、168小时的转录因子C/EBPα、C/EBPβ以及RNA聚合酶II（Pol II）开展时序染色质免疫沉淀测序（ChIP-seq）。此外，还对0小时时间点的IgG阴性对照样本以及转录因子Egr1进行了测序。