Data supporting a study of the prevalence of asymptomatic P. falciparum gametocyte carriage in schoolchildren and assessment of the association between gametocyte density, multiplicity of infection and mosquito infection prevalence

This data record provides details of the data supporting the claims of the related manuscript: “Prevalence of asymptomatic P. falciparum gametocyte carriage in schoolchildren and assessment of the association between gametocyte density, multiplicity of infection and mosquito infection prevalence”.
The data consist of 6 Excel .xlsx format spreadsheets and two Word .docx files.
The related study aimed to assess the prevalence of Plasmodium falciparum gametocyte carriage and some of its associated risk factors among asymptomatic schoolchildren in Western Kenya and further analyse the association between gametocyte density, multiplicity of infection (MOI) and mosquito infection prevalence.

The following files are the data underlying the manuscript:

Study participants screening data_1­.xlsx (NB:
Single = P. falciparum only, Mixed = P.
falciparum plus either P. ovale or P.
malariae or both)
Gam density_MOI_Infection prevalence_3.xlsx.
Sampling period_Infection and Gam
prevalence_2.xlsx.
Raw ELISA output data.xlsx
Raw ELISA output data_OD values.xlsx (NB: raw ELISA data set including the corrected optical density values)
Raw genotyping output data.xlsx
The following are extended data:

Supplementary
file Table S1: Microsatellite Genotyping Primer list.docx.
Samping_site_and_period_analysed_data.docx.
The study was
carried out in Mbita, Homa Bay County of Western Kenya. Study participants were
recruited from primary schools primarily within Mbita sub-county (within 50 km
radius of Mbita town). Primary schoolchildren between the ages five and 15 years residing in Homa bay county, Western Kenya were recruited and screened for P. falciparum malaria infection using a rapid diagnostic test (RDT) and microscopy. Whole blood and filter paper dry blood spots were collected from the study participants upon receiving consent from their parents or legal guidance. Accent was obtained from older children prior to enrolment in the study. Ethical approval for this study was obtained from the Kenya Medical Research Institute Scientific and Ethics Review Unit (SERU) (KEMRI/RES/7/3/1). All the experiments were performed in accordance with the relevant guidelines and regulations.
Version 2 was created to add the file 'Raw ELISA output data_OD values.xlsx'.

本数据集记录为相关手稿《学龄儿童无症状恶性疟原虫（Plasmodium falciparum）配子体（gametocyte）携带率及其与配子体密度、感染复数（multiplicity of infection, MOI）和蚊虫感染率关联的评估》提供支撑数据。
本数据集包含6份Excel .xlsx格式电子表格与2份Word .docx格式文档。
本研究旨在评估肯尼亚西部无症状学龄儿童的恶性疟原虫配子体携带率及其部分相关危险因素，并进一步分析配子体密度、感染复数与蚊虫感染率之间的关联。

支撑该手稿的数据集文件如下：
1. Study participants screening data_1.xlsx（注：单感染=仅恶性疟原虫，混合感染=恶性疟原虫合并卵形疟原虫、三日疟原虫或二者均为阳性）
2. Gam density_MOI_Infection prevalence_3.xlsx
3. Sampling period_Infection and Gam prevalence_2.xlsx
4. Raw ELISA output data.xlsx
5. Raw ELISA output data_OD values.xlsx（注：该原始酶联免疫吸附试验（enzyme-linked immunosorbent assay, ELISA）数据集包含校正后的光密度值）
6. Raw genotyping output data.xlsx

以下为拓展数据集：
- 补充文件表S1：Microsatellite Genotyping Primer list.docx（微卫星基因分型引物列表.docx）
- Sampling_site_and_period_analysed_data.docx（采样地点与分析周期数据.docx）

本研究于肯尼亚西部霍马贝县姆比塔地区开展。研究对象主要从姆比塔副县（姆比塔镇周边50公里范围内）的小学招募。研究纳入居住于肯尼亚西部霍马贝县、年龄5至15岁的小学学龄儿童，通过快速诊断检测（rapid diagnostic test, RDT）与显微镜检查筛查其恶性疟原虫疟疾感染情况。在获得受试者家长或法定监护人的知情同意后，研究人员采集了受试者的全血样本与滤纸片干血斑；对于年龄较大的儿童，还需在入组前获得其本人同意。
本研究已获得肯尼亚医学研究所科学与伦理审查委员会（Scientific and Ethics Review Unit, SERU）的伦理批准（批准号：KEMRI/RES/7/3/1）。所有实验操作均严格遵循相关指南与法规要求。
本数据集V2版本新增了文件"Raw ELISA output data_OD values.xlsx"。