Chromatin state transitions in the Drosophila intestinal lineage reveal principles of cell type specification [RNA-seq]

To investigate the chromatin state into an adult stem cell lineage, we generate cell-type specific chromatin state maps in the adult Drosophila intestine and identify principal chromatin state transitions during lineage determination. we profiled the binding sites of five chromatin-associated proteins from which the previously described five major types of chromatin. Comparative chromatin accessibility (ATAC-seq) as well as gene expression (RNA-seq) in Drosophila ISC/EB in control and H1 RNAi conditions

为探究成体干细胞谱系中的染色质状态调控规律，我们构建了成年果蝇（Drosophila）肠道内细胞类型特异性的染色质状态图谱，并鉴定了谱系决定过程中的核心染色质状态转变事件。我们对五种染色质结合蛋白的结合位点进行了分析，借此明确了此前已报道的五种主要染色质类型。此外，我们还在对照与H1 RNA干扰（RNAi）条件下，对果蝇肠干细胞/肠母细胞（ISC/EB）的染色质可及性（ATAC-seq）与基因表达水平（RNA-seq）开展了对比分析。