Investigating cellular signaling proteins and complexes using time-resolved serial crystallography.

For many years members of the Laboratory of Biomolecular Research have been driving the development of synchrotron based serial crystallography including
pioneering static (Nogly et al., 2015 at ESRF, Weinert et al., 2017) and time-resolved experiments (Nogly et al., 2016, Weinert et al., 2019, Skopintsev et al.,
2022, Weinert et al., 2023) at SLS and SwissFEL. With the coming upgrade of the SLS and an expected shutdown of 2 years, we are submitting a BAG continuation proposal
in order to continue our research projects at the ESRF. Our interests span photoactivation and ligand un-/binding in G protein-coupled receptors, kinase activation,
temperature dependence of enzyme activity and multi-domain photosensors. X-ray beam brilliance, experienced beamline staff and the variety of available sample
delivery methods make the ID29 beamline an ideal place to conduct the proposed projects which have already strengthened collaboration between two
centers of European X-ray science.

多年来，生物分子研究实验室（Laboratory of Biomolecular Research）的科研人员一直推动基于同步辐射（synchrotron）的串行晶体学（serial crystallography）技术发展，先后在瑞士光源（Swiss Light Source, SLS）与瑞士自由电子激光（Swiss Free Electron Laser, SwissFEL）上开展了开创性的静态实验（Nogly等，2015年于欧洲同步辐射装置（European Synchrotron Radiation Facility, ESRF）；Weinert等，2017）与时间分辨实验（time-resolved experiments，Nogly等，2016；Weinert等，2019；Skopintsev等，2022；Weinert等，2023）。鉴于瑞士光源即将启动升级工程并预计停机两年，我们提交了BAG延续性申请提案，以期在欧洲同步辐射装置继续推进相关研究项目。我们的研究方向涵盖G蛋白偶联受体（G protein-coupled receptors）中的光激活（photoactivation）与配体结合/解离过程、激酶激活（kinase activation）、酶活性的温度依赖性以及多域光感受器（multi-domain photosensors）相关研究。X射线束亮度（X-ray beam brilliance）、经验丰富的光束线运维团队，以及多样的可用样品递送方法，使得ID29光束线（ID29 beamline）成为开展本次拟议研究项目的理想场所，而这些项目也已加强了欧洲X射线科学两大中心之间的合作。