Differentially expressed genes after miRNA or siRNA transfection in multiple human cancer cell lines. Differentially expressed genes after miRNA or siRNA transfection in multiple human cancer cell lines

To identify differentially expressed genes by anti cancer treatments (microRNAs or siRNAs) in human cancer, several cell lines ( pancreatic cancer, renal cell carcinoma, oral squamous cell carcinoma, prostate cancer and bladder cancer) were subjected to Agilent whole genome microarrays. Overall design: Human cell lines (PANC-1, 786-O, A498, SAS, PC3 and T24) were treated with miRNAs (miR-455-3p, miR-455-5p, miR-451a, miR-144-3p, miR-144-5p, miR-135-5p, miR-135-3p, miR-30a-5p, miR-30a-3p, miR-184, miR-199-3p, miR-107, miR-185-5p, miR-320b and miR-320c), siRNAs (si-PHLDA2).

为鉴定人类癌症中抗癌治疗（微小RNA（microRNAs）或小干扰RNA（siRNAs））诱导的差异表达基因，本研究将源自胰腺癌、肾细胞癌、口腔鳞状细胞癌、前列腺癌及膀胱癌的多种细胞系进行安捷伦（Agilent）全基因组微阵列芯片检测。实验总体设计：将人类细胞系PANC-1、786-O、A498、SAS、PC3及T24分别用miR-455-3p、miR-455-5p、miR-451a、miR-144-3p、miR-144-5p、miR-135-5p、miR-135-3p、miR-30a-5p、miR-30a-3p、miR-184、miR-199-3p、miR-107、miR-185-5p、miR-320b及miR-320c共15种微小RNA，以及小干扰RNA（si-PHLDA2）进行处理。