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Healthcare workers (HCWs) have a significant occupational risk of hepatitis B virus (HBV) infection. Vaccination remains the most effective measure recommended to avert the risk. However, there’s limited information on hepatitis B vaccine uptake rates and the seroprotection status of HCWs, especially in sub-Saharan Africa. This study aimed to assess hepatitis B vaccination status and also seroprotection status of HCWs in three selected public hospitals in Kenya. This was a cross-sectional study carried out among HCWs at Kenyatta National Hospital (KNH), Naivasha and Mbagathi County hospitals. Data on participants’ demographics and hepatitis B vaccination status was collected using an interviewer-guided questionnaire. Blood samples were collected and tested for hepatitis B surface antigen (HBsAg), hepatitis B surface antibodies (anti–HBs), and hepatitis B core antibodies (anti–HBc) using Enzyme Linked Immuno Sorbent Assay technique. Data were analyzed using Statistical Package for the Social Sciences (SPSS) and Graph pad prism. Of the 145 eligible HCWs, 120 (82.8%) were vaccinated, with 77 (53.1%) having received the recommended three doses. Three quarters (108/145) of the vaccinated HCWs were seroprotected (titres ≥10 mIU/ml) against HBV infection, while 16.6% were non–responders (titres <10 mIU/ml). Vaccination with more than two doses and HBV exposure were significantly associated with anti-HBs titre levels (P<0.05). HCWs who received less than 2 doses of the vaccine were 70% less likely to have high anti-HBs titre levels (aOR, 0.3; 95% CI, 0.1–0.8; P = 0.013). Nearly all HCWs were vaccinated against hepatitis B virus. The majority of all HCWs were seroprotected against hepatitis B virus but a number of them had an insufficient immunity to the virus despite vaccination or prior exposure. There’s need to sensitize HCWs and enforce mandatory full vaccination as per the recommended vaccination schedule.

医护人员（Healthcare workers, HCWs）面临较高的乙型肝炎病毒（Hepatitis B virus, HBV）职业感染风险。接种疫苗仍是目前推荐的最有效规避该风险的手段。然而，目前关于医护人员乙肝疫苗接种率及血清保护状态的相关研究数据较为匮乏，在撒哈拉以南非洲地区尤为如此。本研究旨在评估肯尼亚三家选定公立医院中医护人员的乙肝疫苗接种情况与血清保护状态。本研究为横断面研究，研究对象选自肯雅塔国家医院（Kenyatta National Hospital, KNH）、奈瓦沙县医院及姆巴加蒂县医院的医护人员。研究通过访谈式问卷收集了研究对象的人口统计学资料与乙肝疫苗接种情况相关数据。采集血液样本后，采用酶联免疫吸附试验（Enzyme Linked Immuno Sorbent Assay）检测乙型肝炎表面抗原（Hepatitis B surface antigen, HBsAg）、乙型肝炎表面抗体（anti-HBs）及乙型肝炎核心抗体（anti-HBc）。采用社会科学统计软件包（Statistical Package for the Social Sciences, SPSS）与GraphPad Prism软件对数据进行分析。在145名符合纳入标准的医护人员中，120人（82.8%）已接种乙肝疫苗，其中77人（53.1%）完成了推荐的3剂次接种程序。在完成接种的医护人员中，四分之三（108/145）的个体达到了乙肝病毒感染的血清保护标准（抗体滴度≥10 mIU/ml），另有16.6%的个体为无应答者（抗体滴度<10 mIU/ml）。接种剂次超过2剂次以及乙肝病毒暴露史与抗-HBs抗体滴度水平显著相关（P<0.05）。接种剂次少于2剂的医护人员，其抗-HBs抗体滴度达到高水平的可能性降低70%（调整后比值比aOR=0.3；95%置信区间CI：0.1~0.8；P=0.013）。几乎所有医护人员均已接种乙肝病毒疫苗。大部分医护人员均达到了乙肝病毒的血清保护标准，但仍有部分个体即便完成疫苗接种或曾暴露于乙肝病毒，其机体对病毒的免疫力仍不足。因此，有必要加强对医护人员的宣教，并强制要求其按照推荐的接种程序完成全程疫苗接种。