GFP-Rab11A intracellular movement in RSV infected A549 cells
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https://figshare.com/articles/dataset/GFP-Rab11A_intracellular_movement_in_RSV_infected_A549_cells/10255676
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资源简介:
TIFF series of diSPIM datasets, fused and deconvolved, from A549 GFP-Rab11A cells infected with Respiratory Syncytial Virus. Each TIFF file is a full cell volume of 50 planes, with isotropic resolution of 330nm, each .zip file is an independent replicate with at least 1 or 2 cells analyzed for 3D tracking of cytoplasmic foci with 500 time stamps. These data correspond to Supplementary Movie 9 and analysis of these movies provided data presented in Figure 5 and Supplementary Figure 10.
Supplementary Movie 9: A549 GFP-Rab11A cells infected with RFP-expressing RSV.
Figure 5: Effect of viral infection on Rab11A motion: comparing RSV infection with IAV (WSN) infection.
Supplementary Figure 10: Impact of viral infection on the transport of GFP-Rab11A.
本数据集为经融合与反卷积处理的diSPIM数据集(diSPIM)的TIFF格式序列,来源于感染呼吸道合胞病毒(Respiratory Syncytial Virus,RSV)的A549 GFP-Rab11A细胞。每个TIFF文件对应包含50层切片的完整细胞三维容积,各向同性分辨率为330nm;每个.zip压缩文件为一个独立的生物学重复样本,其中至少包含1~2个细胞,用于对胞质灶进行500个时间点的三维追踪分析。本数据集对应补充影片9,对该影片的分析结果即为图5与补充图10中展示的数据。
补充影片9:感染表达红色荧光蛋白(Red Fluorescent Protein,RFP)的RSV的A549 GFP-Rab11A细胞。
图5:病毒感染对Rab11A运动的影响:对比RSV感染与甲型流感病毒(Influenza A Virus,IAV)WSN株感染的差异。
补充图10:病毒感染对GFP-Rab11A转运过程的影响。
创建时间:
2019-11-07



