广东省常见食用蛇蛙类曼氏裂头蚴病的流行病学调查与综合防控技术

曼氏裂头蚴病广泛流行于东亚地区，在我国大多数分省份也流行，特别以广东珠三 角地区最为严重。近年来，我国感染曼氏裂头蚴病的人数有逐年上升的趋势，已成为威胁人体健康的 主要寄生虫病之一。本项目先后得到广东省科技计划、广东省自然科学基金和广东省林业科技创新等 项目支持，历时8年取得系列重大研究成果。鉴定认为，成果达到同类研究国际先进水平。 （1）系统开展了广东省常见食用蛇蛙类曼氏裂头蚴病的流行病学调查与综合防控研究，全面掌握了我省食用蛇蛙类裂头蚴感染情况，证实人体曼氏裂头蚴感染的主要渠道是生食蛇蛙。对广东食用蛇蛙类消费导致人体裂头蚴感染的公共卫生风险进行定性分析，并识别了主要的风险环节。 （2）建立了用于曼氏裂头蚴虫种鉴定、种内遗传多样性有效分子遗传标记。获得了 1 株曼氏裂头蚴广东分离株线粒体全基因组序列，为该虫的相关种系群体遗传研究提供了宝贵素材。建立了曼氏裂 头蚴感染乌梢蛇模型，建立曼氏裂头蚴 ES 抗原 ELISA 检测方法。研究建立了人和蛇蛙类曼氏裂头蚴病综合防控技术，攻克蛇蛙类曼氏裂头蚴病防控迫切需要解决的技术难题或关键技术，为食用蛇蛙类 曼氏裂头蚴病的防控和人类公共卫生管理提供科技支撑 并将野生蛇蛙类曼氏裂头蚴病列入《广东省重点野生动物疫病种类和疫源物种目录》。（3）共发表论文 12 篇，其中SCI论文5篇 出版专著1部 获得第五届梁希青年优秀论文三等 奖1项。

Spirometra mansoni disease is widely prevalent in East Asia, and endemic in most provinces of China, with the most severe cases in the Pearl River Delta (PRD) region of Guangdong Province. In recent years, the number of human Spirometra mansoni infection cases in China has shown a year-on-year increasing trend, and the disease has become one of the major parasitic diseases threatening human health. This project was supported by the Guangdong Provincial Science and Technology Plan, Guangdong Provincial Natural Science Foundation, and Guangdong Provincial Forestry Science and Technology Innovation projects. After 8 years of research, a series of major research achievements were obtained, which were identified as reaching the international advanced level in similar research fields. (1) Systematically conducted epidemiological investigation and comprehensive prevention and control research on Spirometra mansoni disease in common edible snakes and frogs in Guangdong Province. The infection status of Spirometra mansoni in edible snakes and frogs in the province was comprehensively clarified, and it was confirmed that the main route of human Spirometra mansoni infection is consuming raw snakes and frogs. A qualitative analysis was conducted on the public health risk of human Spirometra mansoni infection caused by the consumption of edible snakes and frogs in Guangdong, and the main risk links were identified. (2) Established effective molecular genetic markers for species identification and intraspecific genetic diversity analysis of Spirometra mansoni. The complete mitochondrial genome sequence of one Guangdong isolate of Spirometra mansoni was obtained, providing valuable materials for related phylogenetic and population genetic studies of this parasite. An infection model of Spirometra mansoni in Zaocys dhumnades was established, and an ELISA detection method using excretory-secretory (ES) antigens of Spirometra mansoni was developed. Comprehensive prevention and control technologies for Spirometra mansoni disease in humans, snakes and frogs were established, overcoming the urgent technical bottlenecks and key technologies required for the prevention and control of Spirometra mansoni disease in edible snakes and frogs. These technologies provided scientific and technological support for the prevention and control of Spirometra mansoni disease in edible snakes and frogs and human public health management. In addition, Spirometra mansoni disease in wild snakes and frogs was included in the Catalogue of Key Wildlife Diseases and Pathogenic Reservoir Species in Guangdong Province. (3) A total of 12 papers were published, including 5 SCI-indexed papers; 1 monograph was published; and 1 third prize of the 5th Liangxi Excellent Youth Paper Award was awarded.