DataSheet_3_A Positive Feedback Loop of Long Noncoding RNA LINC00152 and KLF5 Facilitates Breast Cancer Growth.pdf

The long noncoding RNA (lncRNA) LINC00152, also known as CYTOR, displays aberrant expression in various cancers. However, its clinical value and functional mechanisms in breast cancer remain insufficiently understood. Our study found that LINC00152 is significantly upregulated in breast cancer, and that it acts as an indicator of poor survival prognosis. Further studies revealed that LINC00152 knockdown suppresses cell proliferation and tumorigenicity in vitro and in vivo. Mechanistic analyses demonstrated that LINC00152 directly binds to KLF5 protein and increases KLF5 stability. Moreover, LINC00152 is also a KLF5-responsive lncRNA, and KLF5 activates LINC00152 transcription by directly binding to its promoter. Our study suggests that LINC00152 promotes tumor progression by interacting with KLF5. LINC00152 may be a valuable prognostic predictor for breast cancer, and the positive feedback loop of LINC00152-KLF5 could be a therapeutic target in pharmacological strategies.

长链非编码RNA（long noncoding RNA, lncRNA）LINC00152（亦称CYTOR）在多种癌症中呈现异常表达。然而，其在乳腺癌中的临床价值与功能机制仍未得到充分阐明。本研究发现，LINC00152在乳腺癌组织中显著上调，且可作为不良生存预后的标志物。进一步研究显示，敲低LINC00152可在体外（in vitro）与体内（in vivo）抑制细胞增殖与致瘤性。机制分析表明，LINC00152可直接结合KLF5蛋白并增强其稳定性。此外，LINC00152同时也是KLF5响应性长链非编码RNA，KLF5可通过直接结合其启动子区域激活LINC00152的转录。本研究提示，LINC00152可通过与KLF5相互作用促进肿瘤进展。LINC00152有望成为乳腺癌极具价值的预后预测指标，而LINC00152-KLF5正反馈环路或许可作为药理学策略的治疗靶点。