RNA-seq data related to the study "STT3A is essential for Wnt signaling and represents a target for cancers driven by RNF43 deficiency"

HEK293T cells were plated at a density of 1 million cells per well in 6-well plates. After 16 hours of serum starvation, cells were stimulated with R-spondin1, Wnt3a or CHIR-99021 (GSK3i) for 12 hours. Cells were harvested and total total RNA was extracted using HiPure Total RNA Mini Kit (Magen, R4111-03) for RNA-seq analysis.RNA-seq all:RNAseq-1:RNA-seq data from STT3A-WT HEK293T cells with or without RSPO1 treatment<br>RNAseq-2RNA-seq data from STT3A-KO HEK293T cells with or without RSPO1 treatmentRNAseq-3RNA-seq data from STT3A-WT HEK293T cells with or without Wnt3a treatmentRNAseq-4RNA-seq data from STT3A-KO HEK293T cells with or without Wnt3a treatmentRNAseq-5RNA-seq data from STT3A-WT HEK293T cells with or without GSK3i treatmentRNAseq-6RNA-seq data from STT3A-KO HEK293T cells with or without GSK3i treatment

将HEK293T细胞以每孔1×10⁶个细胞的密度接种于6孔板中。经16小时血清饥饿处理后，分别用R-spondin1、Wnt3a或CHIR-99021（糖原合成激酶3抑制剂，GSK3i）刺激细胞12小时。收集细胞后，使用HiPure Total RNA Mini Kit（Magen，货号R4111-03）提取总RNA，用于RNA测序（RNA-seq）分析。
全部RNA-seq数据详情如下：
RNAseq-1：STT3A野生型（STT3A-WT）HEK293T细胞经或未经RSPO1处理的RNA-seq数据
RNAseq-2：STT3A基因敲除型（STT3A-KO）HEK293T细胞经或未经RSPO1处理的RNA-seq数据
RNAseq-3：STT3A-WT HEK293T细胞经或未经Wnt3a处理的RNA-seq数据
RNAseq-4：STT3A-KO HEK293T细胞经或未经Wnt3a处理的RNA-seq数据
RNAseq-5：STT3A-WT HEK293T细胞经或未经GSK3i处理的RNA-seq数据
RNAseq-6：STT3A-KO HEK293T细胞经或未经GSK3i处理的RNA-seq数据