Corpus callosum bulk RNA sequencing of a cuprizone mouse model of demyelination

We used the cuprizone mouse model of multiple sclerosis to determine whether Bruton's tyrosine kinase (BTK) inhibition could attenuate disease-relevant changes to central nervous system gene expression. Overall design: The corpus callosum was dissected from the brains of a cuprizone mouse model of demyelination. Bulk RNA sequencing was then performed. A subset of mice had been treated with an inhibitor of Bruton's tyrosine kinase (BTK) after disease induction.

本研究采用多发性硬化铜嗪小鼠模型，旨在探究布鲁顿酪氨酸激酶（Bruton's tyrosine kinase, BTK）抑制是否可缓解中枢神经系统基因表达的疾病相关性改变。实验整体设计：从脱髓鞘铜嗪小鼠模型的大脑中解剖分离胼胝体，随后开展批量RNA测序。其中部分小鼠在疾病造模完成后，接受了布鲁顿酪氨酸激酶抑制剂处理。