Differentially expressed lncRNA&mRNA between macrophage M0 and macrophage M2 cells. Differentially expressed lncRNA&mRNA between macrophage M0 and macrophage M2 cells

To compare lncRNA&mRNA expression diffrence between macrophage M0 and macrophage M2 cells, we performed microarray using Arraystar Human LncRNA + mRNA Microarray v3.0 (8×60K) platform Overall design: Total RNAs from six independent groups of M0 macrophages or M2 macrophages were isolated using Trizol reagent (Invitrogen life technologies, CA, USA),and labeled using Quick Amp Labeling Kit, One-Color (Agilent p/n 5190-0442). Next, the labeled/amplified RNA and labeled cRNA was purified and hybridizated using Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242). Microarray slides were washed and scanned on an Agilent Microarray Scanner (Agilent p/n G2565BA), and data were extracted using Agilent Feature Extraction Software.

为比较巨噬细胞M0与巨噬细胞M2细胞之间的长链非编码RNA（lncRNA）和信使RNA（mRNA）表达差异，我们采用Arraystar人类长链非编码RNA+信使RNA微阵列v3.0（8×60K）平台开展微阵列实验。
实验整体设计：我们从6组独立的M0巨噬细胞和M2巨噬细胞样本中提取总RNA，使用Trizol试剂（Invitrogen生命技术公司，美国加利福尼亚州）完成提取，并通过Quick Amp单色标记试剂盒（安捷伦，货号5190-0442）进行标记。随后，将标记并扩增后的RNA与标记好的互补RNA（cRNA）进行纯化，采用安捷伦基因表达杂交试剂盒（安捷伦，货号5188-5242）完成杂交。微阵列芯片经洗涤后，在安捷伦微阵列扫描仪（安捷伦，货号G2565BA）上进行扫描，并通过安捷伦特征提取软件完成数据提取。