Transcriptome Sequencing and Assembly of Canna indica Flower. Canna indica

Canna indica L. is an ornamental plant with petaloid staminodes and only a half fertile stamen in its flowers. The genetic basis for petaloid androecium remains unclear. In order to get comprehensive transcriptome data for further studies, RNA-Seq analysis were carried out. Two libraries from flower primordia and differentiated flowers of Canna indica were constructed and sequenced respectively, and totally 118,869 unigenes were assembled. The unigenes were aligned to the protein databases NR, NT, Swiss-Prot, KEGG, COG and GO (e-value<0.00001), and totally 67,299 unigenes were annotated. Our data constitute a preliminary basis for further studies on flower development of Canna indica. Overall design: The two samples from flower primordium and differentiated flower were sequenced for transcriptome assembly, and gene expression information of the two stages was also obtained from these data.

美人蕉（Canna indica L.）是一类花器官中具花瓣状退化雄蕊且仅含半枚可育雄蕊的观赏植物。目前其瓣化雄蕊群的遗传基础仍未明确。为获取可支撑后续研究的全面转录组数据，本研究开展了RNA测序（RNA-Seq）分析。我们分别构建并测序了取自美人蕉花原基与分化完成花的两个文库，最终组装得到118869条单基因簇（unigenes）。将所获单基因簇（unigenes）比对至NR、NT、Swiss-Prot、KEGG、COG及GO蛋白数据库（E值<0.00001），最终共有67299条单基因簇获得功能注释。本研究所得数据可为美人蕉花发育相关的后续研究提供初步基础。整体实验设计：本研究对花原基与分化花两个样本进行测序以完成转录组组装，同时从上述测序数据中获取了两个发育阶段的基因表达信息。