Microbiome assembly of the diatom Asterionellopsis glacialis

The multitude of interactions between bacterioplankton and phytoplankton have prompted numerous studies that investigate phytoplankton microbiomes with the aim of characterizing beneficial or opportunistic taxa and elucidating core bacterial members. Often times, this knowledge is garnered through 16S rRNA gene profiling over time and across locations. In the laboratory, phytoplankton cultures are typically maintained in synthetic, nutrient rich media while may skew our understanding of the microbiome. In this study, we aimed to identify taxa that are central to structuring and establishing the overall microbiome of the diatom Asterionellopsis glacialis. We introduced a diverse environmental bacterial community to Asterionellopsis glacialis in a continuous culture set-up in nutrient-rich and nutrient-poor media, and profiled the bacterial community over a period of 7 days.

浮游细菌与浮游植物间的广泛互作，催生了诸多针对浮游植物微生物组的研究，旨在解析有益或机会性类群，并阐明核心细菌成员。通常而言，这类研究认知多通过跨时空的16S rRNA基因谱分析获得。实验室环境中，浮游植物通常培养于合成富营养培养基中，但该培养条件可能会扭曲我们对其微生物组的真实认知。本研究旨在明确对硅藻冰缘斜纹藻（Asterionellopsis glacialis）整体微生物组的构建与形成起核心作用的类群。我们通过富营养与贫营养两种培养基的连续培养体系，将多样化的环境细菌群落接种至该硅藻体系中，并对为期7天内的细菌群落进行了动态谱分析。