Stable overexpression of IRF8 in hepatocellular carcinoma cells hepa1-6 xenograft derived tumors in C57bl/6 mice

Interferon regulatory factor 8 (IRF8), also known as interferon consensus sequence-binding protein, is an essential lineage-specific transcriptional regulator in the IRF family, controls diverse cellular processes and is has been implicated in innate immunity, immune cell differentiation. IRF8 has been shown to have both oncogenic and tumor suppressor activities, in myeloid cells and nonhematopoietic cells. As a transcription factor, other functions which may contribute to its oncogenic and anti tumor potential are not clear. So it is meaningful to study the role of IRF8 in different diseases. The roles and the underlying mechanisms of intrahepatic IRF8 in Hepatocellular carcinoma have not been reported up to date. Overall design: mRNA profile of Hepatocarcinoma cell line Hepa1-6 with IRF8-overexpression and vector control xenografted into C57bl/6 mice were generated by deep sequencing

干扰素调节因子8（Interferon regulatory factor 8, IRF8），亦称干扰素共有序列结合蛋白（interferon consensus sequence-binding protein），是IRF家族中一类关键的谱系特异性转录调控因子。其可调控多种细胞进程，并与先天免疫、免疫细胞分化过程密切相关。已有研究证实，IRF8在髓系细胞与非造血细胞中均兼具致癌活性与抑癌活性。作为一类转录因子，其促癌与抗肿瘤潜能背后的其他潜在功能机制尚不明确，因此探究IRF8在不同疾病中的作用具有重要研究价值。截至目前，肝内IRF8在肝细胞癌（Hepatocellular carcinoma, HCC）中的作用及潜在机制尚未见诸报道。本实验整体设计为：通过深度测序技术，获取IRF8过表达组与空载对照组的肝癌细胞系Hepa1-6异种移植至C57BL/6小鼠体内的mRNA表达谱。