Bulk RNA-seq of skeletal muscle reveals the role of ZBED6 in sepsis-induced muscle atrophy in minipigs

Sepsis is a severe disease and induces skeletal muscle atrophy, which has a significant impact on patients. Moreover, the onset and severity of muscle atrophy can vary across different anatomical regions during sepsis. Our previous study demonstrated that ZBED6 knockout not only promotes skeletal muscle growth under physiological conditions, but also alleviates systemic muscle atrophy during sepsis. However, its region-specific effects on sepsis-induced muscle atrophy have not been analyzed. In this study, we performed RNA sequencing for 54 samples of skeletal muscle from nine different anatomical regions from male ZBED6 knockout and wild-type (WT) minipigs subjected to sepsis. We generated 364.63 Gb of high-quality bulk RNA sequencing data from the skeletal muscle samples (approximately 6.75 Gb per sample). This dataset provided insightful gene expression information to understand the role of ZBED6 in region-specific muscle atrophy during sepsis. In addition, this dataset can be used for heterogeneity analysis between skeletal muscle tissues from multiple species or female pigs.

脓毒症（Sepsis）是一种重症疾病，可诱发骨骼肌萎缩，对患者造成显著影响。此外，脓毒症病程中，肌肉萎缩的发生与严重程度在不同解剖部位间存在差异。我们此前的研究证实，ZBED6基因敲除（ZBED6 knockout）不仅可在生理条件下促进骨骼肌生长，还能缓解脓毒症引发的全身性肌肉萎缩，但目前尚未有研究分析其对脓毒症诱导的肌肉萎缩的部位特异性调控效应。本研究针对雄性ZBED6基因敲除与野生型（WT）迷你猪在脓毒症状态下的9个不同解剖部位的54份骨骼肌样本开展了RNA测序（RNA sequencing），最终从这批骨骼肌样本中获得了总计364.63 Gb的高质量批量RNA测序数据，单样本平均测序数据量约为6.75 Gb。本数据集提供了具有参考价值的基因表达信息，可为解析ZBED6在脓毒症期间部位特异性肌肉萎缩中的作用提供洞见，此外该数据集还可用于跨多个物种的骨骼肌组织间或雌性猪骨骼肌组织的异质性分析（heterogeneity analysis）。