Processed HLA-A allele calls

This dataset contains processed HLA-A genotyping results and accompanying clinical metadata from a study of 64 Ugandan people living with HIV (32 periodontitis cases and 32 healthy controls), nested within the Oral Papilloma Virus Microbiota and Cancer (OHPVMC) parent study. The genetic component features HLA-A allele calls in CSV format, generated through nanopore sequencing of exons 2-3 using Oxford Nanopore technology, with sequences aligned to the IMGT/HLA reference database. The clinical metadata includes comprehensive periodontal health assessments (WHO CPI criteria), demographic information (age, sex, education, occupation), HIV treatment history (ART duration), and behavioral factors (smoking, alcohol use).All samples underwent standardized processing, including DNA extraction from buffy coats and library preparation using Oxford Nanopore's Rapid Barcoding protocol. The dataset provides a unique resource for investigating host genetic factors in periodontitis susceptibility among HIV-positive African populations, while also serving as a reference for nanopore-based HLA typing methodologies.

本数据集包含经过标准化处理的人类白细胞抗原A（HLA-A）基因分型结果，以及配套的临床元数据，其源自一项针对64名乌干达人类免疫缺陷病毒（HIV）感染者的研究——其中32例为牙周炎患者，32例为健康对照，该研究嵌套于口腔乳头瘤病毒菌群与癌症（OHPVMC）母体研究之中。该数据集的遗传组分以CSV格式存储HLA-A等位基因分型结果，通过牛津纳米孔（Oxford Nanopore）技术对外显子2-3进行纳米孔测序获得，测序序列均比对至IMGT/HLA参考数据库。临床元数据涵盖全面的牙周健康评估（采用世界卫生组织社区牙周指数（WHO CPI）判定标准）、人口统计学信息（年龄、性别、受教育程度、职业）、HIV治疗史（抗逆转录病毒治疗（ART）时长）以及行为相关因素（吸烟、饮酒情况）。所有样本均经过标准化流程处理，包括从血沉棕黄层中提取基因组DNA，以及采用牛津纳米孔的快速条形码建库协议完成文库制备。本数据集为探究HIV阳性非洲人群牙周炎易感性的宿主遗传因素提供了独特的研究资源，同时也可作为基于纳米孔技术的HLA分型方法的参考数据集。