Analysis of PIK3CA, AKT1 and pAKT T308 in Merkel cell carcinoma.

Age, age at the time of diagnosis; m, male; f, female; MCV, Merkel cell polyoma virus (the MCV status was assessed as described previously [2]); wt, wild-type; wt*, these samples could not be sequenced directly, but were analyzed by a modified SNaPshot® assay; −, not available; IHC pAKT T308, immunohistochemistry for phospho-AKT threonine 308 scored from 0 (negative) to 3+ (very strong) staining intensity; it has to be noted that only 9 samples used for mutation analysis were also present on the tissue microarray.

年龄：诊断时的年龄；m代表男性（male），f代表女性（female）；MCV即默克尔细胞多瘤病毒（Merkel cell polyoma virus），其状态评估方法详见此前文献[2]；wt为野生型（wild-type）；wt*：此类样本无法直接进行测序，需通过改良的SNaPshot®检测技术完成分析；符号“−”表示数据不可用；IHC pAKT T308：针对磷酸化AKT苏氨酸308位点的免疫组化检测，染色强度按0（阴性）至3+（极强）进行分级评分；需注意：仅9份用于突变分析的样本同时存在于组织微阵列中。