Additional file 2: of Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves

Table S1. Differentially accumulated transcripts (DATs) upon MRCV infection at 12 and 21 dpi. For each gene, the normalized mean count for mock-inoculated and MRCV-infected samples, the fold change and the FDR-adjusted P-value (padj) are indicated. The annotations from Ensembl plants Release 37 or inferred in this work are included. Table S2. DATs classified as wheat Transcription factors according to reference [27]. Table S3. DATs classified as Carbohydrate-related according to their functional annotation. Table S4. Putative wheat invertases and fructosyltransferases, Tre6P synthases (TPS) and Sugars Will Eventually be Exported Transporters (SWEET) used to build the phylogenetic trees shown on Additional file 1: Figures S4-S6. Sequences encoded by DATs at 21 dpi are highlighted in red (increased upon the infection) and blue (decreased). A/N Inv, Vac Inv and CW Inv stand for acid and/or neutral, vacuolar and cell wall invertases respectively. Fructosyltransferases included sucrose:sucrose 1-fructosyltransferase (1-SST), fructan:fructan 1-fructosyltransferases (1-FFT) and sucrose:fructan 6-fructosyltransferases (6-SFT). Table S5. DATs classified as Amino acid-related according to their functional annotation. Amino acid transporters were extracted from reference [45]. Table S6. DATs classified as Hormone-related according to their functional annotation. Table S7. Primer sequences used for qRT-PCR validations. (XLSX 717 kb)

附表S1：MRCV侵染后12和21天（dpi，接种后天数）的差异积累转录本（Differentially accumulated transcripts，简称DATs）。针对每个基因，均标注了空白对照接种组与MRCV侵染组样本的标准化平均计数、倍数变化值，以及经错误发现率（False Discovery Rate，简称FDR）校正后的P值（padj）。本研究纳入了植物Ensembl数据库第37版（Ensembl plants Release 37）的注释信息，或本研究推导得到的注释。
附表S2：依据参考文献[27]归类为小麦转录因子的DATs。
附表S3：依据功能注释归类为碳水化合物相关的DATs。
附表S4：用于构建附加文件1中图S4~S6所示系统发育树的推定小麦蔗糖酶、果聚糖转移酶、海藻糖-6-磷酸合酶（Tre6P synthases，简称TPS）以及糖最终输出转运蛋白（Sugars Will Eventually be Exported Transporters，简称SWEET）。其中，接种后21天的DATs所编码的序列分别以红色（侵染后表达上调）和蓝色（侵染后表达下调）标注。A/N Inv、Vac Inv及CW Inv分别指代酸性/中性蔗糖酶、液泡蔗糖酶以及细胞壁蔗糖酶。果聚糖转移酶涵盖蔗糖:蔗糖1-果聚糖转移酶（1-SST）、果聚糖:果聚糖1-果聚糖转移酶（1-FFT）以及蔗糖:果聚糖6-果聚糖转移酶（6-SFT）。
附表S5：依据功能注释归类为氨基酸相关的DATs。氨基酸转运蛋白信息提取自参考文献[45]。
附表S6：依据功能注释归类为激素相关的DATs。
附表S7：用于qRT-PCR验证的引物序列。（XLSX 717 kb）