RNA-Seq analysis LEF1 or KDM4A knockdown effect on CAL-27 cells. RNA-Seq analysis LEF1 or KDM4A knockdown effect on CAL-27 cells

To investigate the carcinogenesis function LEF1 or KDM4A in the regulation of oral squamous cell carcinoma, we used siRNA knockdown of LEF1 or KDM4A in CAL-27 cells. Overall design: CAL-27 cells were transfected with siLEF1, siKDM4A or control (n=3 for each group) for 48 hours. Total RNA was isolated using Trizol reagent (Invitrogen, USA). Agilent 2100 Bioanalyzer (Agilent Technologies, USA) was used to evaluate the RNA quality.

为探究LEF1与KDM4A在口腔鳞状细胞癌调控过程中发挥的致癌功能，我们在CAL-27细胞中采用小干扰RNA（siRNA）敲低LEF1或KDM4A的表达。
实验整体设计：将CAL-27细胞分别转染siLEF1、siKDM4A或阴性对照（每组n=3），培养48小时后，使用Trizol试剂（美国Invitrogen公司）提取总RNA。采用安捷伦2100生物分析仪（Agilent Technologies，美国）对RNA质量进行评估。