Cell cycle of yeast deleted for yox1

Transcription profiles across the cell cycle of yox1 deleted yeast cells. BY2399 cells (yox1 delete cells isogenic with W303a) were arrested with alpha factor in YEPD media. After release from arrest cells were sampled every 5 min for 2 hr. Keywords: cell cycle time course Cells were synchronized with alpha factor and sampled every 5 min across 2 cell cycles. A total of 25 samples were analyzed. Technical replicates were included. cDNA of the cell cycle samples were labelled with Cy5. For Cy3 labelling, asynchronous yeast population was used.

YOX1基因缺失酵母细胞的细胞周期转录谱。BY2399细胞（即与W303a同源的YOX1缺失细胞）于YEPD培养基中利用α因子进行细胞周期阻滞。解除阻滞后，每5分钟采集一次细胞样本，持续2小时。关键词：细胞周期时间序列实验。本研究通过α因子同步化细胞，并在2个细胞周期内每5分钟采集一次样本，共计分析25份样本，且设置了技术重复。细胞周期样本的互补DNA（cDNA）采用Cy5进行荧光标记；Cy3荧光标记则以异步生长的酵母群体作为参照样本。