RNA-seq of Mycobacterium smegmatis mc2 155 negative control strain (NC), carD (MSMEG_6077) and crsL (MSMEG_5890) knockdown strains, from exponential and stationary phase cells.

CarD is an essential transcription factor for Mycobacterium smegmatis. We identified a new interaction partner of CarD and named it CrsL (MSMEG_5890). To address the genome-wide effect of CrsL, we generated knockdown strains for both carD (MSMEG_6077) and crsL (MSMEG_5890) genes in Mycobacterium smegmatis. We aimed to compare the differentially expressed genes of crsL knockdown and correlate them with the ones of carD knockdown to describe the role of CrsL in transcription.

CarD是耻垢分枝杆菌（Mycobacterium smegmatis）的必需转录因子。本研究鉴定得到CarD的一种新型互作伴侣蛋白，并将其命名为CrsL（MSMEG_5890）。为探究CrsL在全基因组层面的调控效应，我们分别构建了耻垢分枝杆菌中carD（MSMEG_6077）与crsL（MSMEG_5890）基因的敲低菌株。本研究旨在对比crsL敲低菌株的差异表达基因，并将其与carD敲低菌株的差异表达基因进行关联分析，以阐明CrsL在转录过程中的功能。