Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool105_h1930004_Ig

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snmC-seq3 to brain regions to identify epigenomic landcape of the adult human brains

阐明人脑的细胞架构，对于理解其多样功能与复杂动物行为至关重要。本研究对人脑多个区域应用单细胞核甲基化测序（single nucleus methylation sequencing）技术，鉴定得到多个特征鲜明的细胞簇，并构建了层级分类体系。这些细胞簇涵盖了已报道的主要脑细胞类型以及潜在亚型。本研究利用该数据集鉴定各细胞簇的表观基因组特征，并明确其特异性调控元件。实验整体设计：对脑区应用单细胞核甲基化测序3.0（snmC-seq3）技术，以解析成年人脑的表观基因组全貌。