Effects of GK and MK peptides on DENV growth.
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https://figshare.com/articles/dataset/_Effects_of_GK_and_MK_peptides_on_DENV_growth_/477994
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C6/36 cells were infected with DENV (serotypes 1–4) at a MOI of 1 and were cultured in the absence (PC) or the presence of 10 µg/ml of each of the GK, MK or the control MT peptides. After 24 h of culture, supernatants were collected and the presence of DENV was determined by plaque assay. Plaque Forming Units/ml in cell culture supernatant are presented as the mean of four replicates from two independent experiments.
以感染复数(MOI)为1的登革病毒(DENV,血清型1至4)感染C6/36细胞,随后分别在不含(PC)或添加10 μg/ml GK、MK及对照MT多肽的条件下进行培养。培养24小时后收集细胞培养上清液,通过噬斑实验检测登革病毒的存在量。细胞培养上清液中的噬斑形成单位(PFU)/mL以两次独立实验的四次重复样本的均值进行呈现。
创建时间:
2011-01-13



