Rice genome-scale network integration reveals transcriptional regulators of grass cell wall synthesis

Grasses have evolved distinct cell wall composition and patterning relative to dicotyledonous plants. However, despite the importance of this plant family, transcriptional regulation of its cell wall biosynthesis is poorly understood. To identify grass cell wall-associated transcription factors, we constructed the Rice Combined mutual Ranked Network (RCRN). The RCRN covers >90% of annotated rice (Oryza sativa) genes, is high quality, and includes most grass-specific cell wall genes, such as mixed-linkage glucan synthases and hydroxycinnamoyl acyltransferases. Comparing the RCRN and an equivalent Arabidopsis network suggests that grass orthologs of most genetically verified eudicot cell wall regulators also control this process in grasses, but some vary significantly in network connectivity between these divergent species. Reverse genetics, yeast-one-hybrid, and protoplast-based assays reveal that OsMYB61a activates a grass-specific acyltransferase promoter, which confirms network pre...

与双子叶植物相比，禾本科植物的细胞壁组成和模式已进化出显著差异。然而，尽管该科植物具有重要意义，其细胞壁生物合成的转录调控机制仍知之甚少。为鉴定禾本科植物细胞壁相关转录因子，我们构建了水稻联合互排名网络（Rice Combined mutual Ranked Network, RCRN）。RCRN覆盖了90%以上的已注释水稻（Oryza sativa）基因，质量较高，且包含大多数禾本科特有细胞壁基因，例如混合连接葡聚糖合酶和羟基肉桂酰酰基转移酶。通过比较RCRN与等效的拟南芥（Arabidopsis）网络发现，大多数经遗传验证的真双子叶植物细胞壁调控因子的禾本科直向同源物，在禾本科植物中也调控该过程，但部分调控因子在这两个差异物种间的网络连接性存在显著差异。反向遗传学、酵母单杂交和原生质体实验表明，OsMYB61a可激活禾本科特有酰基转移酶启动子，这证实了网络预...