Multilocus Sequence Typing (MLST) scheme for molecular typing of Trueperella pyogenes

Trueperella pyogenes is a Gram-positive bacterium causing purulent opportunistic infections in livestock and wild animals. Despite its broad host distribution and significant economic impact on the livestock industry, the global diversity and population structure of T. pyogenes remain poorly characterised. The aim of this study was to develop a multilocus sequence typing (MLST) scheme for T. pyogenes. This scheme was developed based on the sequences of seven housekeeping genes (adk, gyrB, leuA, metG, recA, tpi and tuf) and applied to study data for 114 T. pyogenes isolates. This scheme is a solid frame for differentiation and phylogenetic analysis of T. pyogenes isolates, which will help to improve our understanding of the epidemiology of infections caused by this pathogen.Data deposited in the repository was saved in .fasta format.

化脓隐秘杆菌（Trueperella pyogenes）是一种革兰氏阳性菌，可引发畜禽与野生动物的化脓性机会性感染。尽管该菌宿主分布范围广泛且对畜禽养殖业造成显著经济影响，但目前学界对化脓隐秘杆菌的全球多样性与种群结构仍缺乏充分解析。本研究旨在为化脓隐秘杆菌建立一套多位点序列分型（multilocus sequence typing，MLST）方案。该方案基于7个持家基因（adk、gyrB、leuA、metG、recA、tpi及tuf）的序列开发，并应用于114株化脓隐秘杆菌分离株的研究分析。该方案可作为化脓隐秘杆菌分离株分型与系统发育分析的坚实框架，有助于深化我们对该病原菌所致感染流行病学的认知。存储于公共数据库的相关数据均以.fasta格式保存。