Rabbit Model of Fetal Tracheal Occlusion for Congenital Diaphragmatic Hernia. Oryctolagus cuniculus

Analysis of embronic day 30.5 (E30.5) fetal rabbit left lung after creation of diapragmatic hernia (DH) at E25 and tracheal occlusion (TO) at E27. Overall design: The fetuses of timed pregnant New Zealand rabbits were subjected to creation of a left diaphragmatic hernia at E25 with or without tracheal occlusion at E27. At E30.5, the fetuses were sacrificed with collection of the lateral aspect of the left upper lobe for NextGen mRNA sequencing.

本研究针对胚胎第30.5天（E30.5）的胎兔左肺展开分析，该胎兔模型于胚胎第25天（E25）构建左侧膈疝（diaphragmatic hernia, DH），并于胚胎第27天（E27）实施气管闭塞术（tracheal occlusion, TO）。实验整体设计如下：选取经定时交配确认孕龄的新西兰兔孕体，对其胎兔于E25日龄构建左侧膈疝，设置是否在E27日龄实施气管闭塞术的对照分组；于E30.5日龄处死所有胎兔，采集左肺上叶外侧组织样本，用于下一代mRNA测序（NextGen mRNA sequencing）。