Sample Dataset for SIM/STORM Correlation

Final SIM Reconstruction.tif
Tubulin-stained HeLa cell reconstructed from 3D SIM acquisition.
The 3D stack was then Maximum-Projected

Final STORM Reconstruction.tif
Result from ThunderSTORM analyis with astigmatism, projected in 2D

SIM and STORM Folders
Contains a series of raw bead images acquired on the same microscope using either the SIM optics or the STORM optics. In our case, the difference between the two comes from the presence of a 2.5X lens when in SIM mode and a 1.0X lens with astigmatism when in STORM mode.
The images need to have the same name in both folders in order for them to open properly

最终结构光照明显微镜（Structured Illumination Microscopy, SIM）重建图像.tif
经微管蛋白染色的海拉细胞（HeLa cell），其成像数据通过三维结构光照明显微镜采集后完成重建。
随后对该三维图像栈进行最大强度投影处理。

最终随机光学重建显微镜（Stochastic Optical Reconstruction Microscopy, STORM）重建图像.tif
该结果通过采用像散成像模式的ThunderSTORM软件分析得到，已完成二维投影。

SIM与STORM数据集文件夹
该文件夹包含一系列在同一台显微镜上采集的微球原始图像，采集时分别启用SIM光学通路或STORM光学通路。在本实验中，两种光学通路的差异在于：SIM模式下搭载2.5倍物镜，STORM模式下则搭载1.0倍物镜并启用像散成像。
为确保图像能够正常加载，两个文件夹内的图像文件名需完全一致。