Gene expression profiles of Vibrio parahaemolyticus in response to curcumin or epigallocatechin gallate

Vibrio parahaemolyticus RIMD2210633 was incubated in Luria-Bertani (LB) broth, composed of 1 percent Oxoid tryptone, 0.5 percent Oxoid yeast extract, and 1 percent NaCl, at 37 degrees with shaking at 200 rpm overnight. Then, the cell culture was diluted 1:50 into 5 ml of LB broth for a second round of growth, reaching an optical density at 600 nm value of 1.4. For the third round of growth, the culture was diluted 1:1000 into 5 ml of LB broth and harvested during the mid-exponential growth phase (OD600=1.4). Bacterial cells cultured in LB broth supplemented with 25 micrograms per milliliter curcumin or 4 mM epigallocatechin gallate (EGCG) were designated as the experimental group, while those cultured in LB broth without curcumin or EGCG were designated as the control group. This setup was used to investigate the effects of sublethal doses of curcumin or EGCG on gene expression in V. parahaemolyticus using RNA-seq.

副溶血性弧菌（Vibrio parahaemolyticus）RIMD2210633菌株于Luria-Bertani（LB）肉汤中培养，该肉汤由1% Oxoid胰蛋白胨、0.5% Oxoid酵母提取物及1%氯化钠组成，培养条件为37℃、200 rpm振荡过夜。随后将菌液以1:50的比例稀释至5 mL新鲜LB肉汤中进行二次培养，直至600 nm波长下的光密度（OD₆₀₀）达到1.4。第三次培养时，将菌液以1:1000的比例稀释至5 mL LB肉汤中，并于指数生长中期（OD₆₀₀=1.4）收集菌体。分别使用添加25 μg/mL姜黄素或4 mM表没食子儿茶素没食子酸酯（epigallocatechin gallate, EGCG）的LB肉汤培养的菌体作为实验组，以未添加姜黄素及EGCG的LB肉汤培养的菌体作为对照组。本实验体系旨在通过RNA测序（RNA-seq）探究亚致死剂量的姜黄素或EGCG对副溶血性弧菌基因表达的影响。