Glyphosate on shikimic acid on Urochloa plantaginea

ABSTRACT: Shikimic acid (SA) has witnessed a strong increase in recent years due to the increasing demand of the pharmaceutical and cosmetic industry. The SA is used as a precursor for the synthesis of oseltamivir phosphate (Tamiflu®), a potent viral inhibitor and is extracted from the plant Illicium verum Hook which has a limited availability. This article proposed the use of Urochloa plantaginea (Link.) webster and glyphosate, as an alternative source of SA. U. plantaginea plants with 3 - 4 tillers and 4 - 6 leaves were harvest at 3, 6, 9 and 12 days after application (DAT) of low rates of glyphosate. Samples were dried, extracted, analyzed by HPLC and LC-MS/MS. The maximum SA concentrations were observed at 6 days after glyphosate at 36 g.a.e.ha-1 was applied in plants of U. plantaginea with 4 to 6 leaves. The capability of this annual gramineae to produce elevated SA levels throughout the entire biomass affords its potential for a greater yield on a per hectare basis.

摘要：莽草酸（Shikimic acid, SA）近年来随着医药与化妆品行业需求的持续增长，市场需求量显著攀升。莽草酸是合成磷酸奥司他韦（oseltamivir phosphate, Tamiflu®）的前体原料，后者是一种强效病毒抑制剂，传统上从八角茴香（Illicium verum Hook）中提取，但该植物的资源供应量有限。本文提出采用类黍尾稃草（Urochloa plantaginea (Link.) webster）与草甘膦（glyphosate）作为莽草酸的替代来源。研究选取具备3~4个分蘖、4~6片叶片的类黍尾稃草植株，在施加低剂量草甘膦后的第3、6、9、12天（DAT）进行采收。将采集的样品经干燥、萃取后，采用高效液相色谱（High Performance Liquid Chromatography, HPLC）与液相色谱-串联质谱（Liquid Chromatography-tandem Mass Spectrometry, LC-MS/MS）进行检测分析。结果表明，当向拥有4~6片叶片的类黍尾稃草植株施加36 g.a.e.ha⁻¹剂量的草甘膦后，施药后第6天测得的莽草酸浓度达到峰值。该一年生禾本科植物能够在全株生物量中积累高浓度莽草酸，这为其实现更高的单位公顷产量提供了应用潜力。