Selective depletion of cancer cells with extrachromosomal DNA via lentiviral infection

We observed depletion of cells containing ecDNA during lentiviral but not transposon-based transduction. This discovery may provide critical information on utilizing a lentiviral system in emerging ecDNA research. Additionally, this observation suggests specific sensitivities for cells with ecDNA.

我们观察到，在慢病毒转导过程中，携带染色体外DNA（extrachromosomal DNA, ecDNA）的细胞出现了耗竭现象，而基于转座子的转导则未出现该情况。这一发现可为新兴的染色体外DNA研究中慢病毒系统的应用提供关键参考依据。此外，该观测结果表明携带染色体外DNA的细胞具有特定的敏感性。