Single cell RNA seq analysis of postnatal mouse utricle

Undamaged utricular sensory epithelia from P2, P4, and P6 mice were isolated using FACS and underwent Smartseq-2 protocol for single cell RNA seq. Data was generated using Illumina NextSeq 500, paired end, 75-150bp reads with median depth of ~0.5 million reads per cell. Overall design: FACS sorted mouse utricle cells, Smart-seq2 protocol

本研究采用荧光激活细胞分选（Fluorescence-Activated Cell Sorting，FACS），从P2、P4、P6龄小鼠体内分离得到完整无损的椭圆囊感觉上皮，随后通过Smart-seq2实验流程开展单细胞RNA测序。本次测序数据依托Illumina NextSeq 500测序平台生成，采用双端测序模式，读长范围为75至150碱基对，单细胞中位数测序深度约为50万条读段。实验整体设计：经FACS分选的小鼠椭圆囊细胞，采用Smart-seq2实验流程。