Optimized minimal genome-wide human sgRNA library. Optimized minimal genome-wide human sgRNA library

To minimize the human genome-wide CRISPR/Cas9 library size, we established H-mLib which recruited a novel sgRNA design method and applied with dual plasmid based strategy. The performance of the H-mLib was benchmarked to other CRISPR libraries in a proliferation screening conducted in K562 cells. We also identified human core essential genes and cell-type specific essentials genes in K562 and Jurkat cells. Overall design: CRISPR screen

为缩小人类全基因组CRISPR/Cas9文库的规模，我们构建了H-mLib文库，该文库采用了新型单向导RNA（single guide RNA, sgRNA）设计方法，并应用了基于双质粒的实验策略。我们在K562细胞系中开展增殖筛选实验，将H-mLib的性能与其他CRISPR文库进行基准对比。此外，我们还在K562和Jurkat细胞系中鉴定出人类核心必需基因以及细胞类型特异性必需基因。整体实验设计：CRISPR筛选