microRNA expression profile related to lymph node status in women with stage grade 1-2-3 endometrial cancer

The aim of this study was to evaluate whether microRNA profiles of grade 1 – 2 or 3 endometrioid adenocarcinomas can be related to nodal status and used as a tool to adapt surgical staging in early-stage EC. MicroRNA expression were assessed in twelve formalin-fixed paraffin-embedded (FFPE) EC primary tumors with positive lymph node and in 36 FFPE EC primary tumors with negative lymph node, matched for grade, stage and lymphovascular space involvement status. Total RNA was extracted using the miRNeasy FFPE Kit (Qiagen, Courtaboeuf, France), according to the manufacturer’s instructions. The 36 specimens of negative LN status were pooled by 3 in accordance with the matching criteria. Microarray analysis was conducted on 48 distinct specimens: Twelve specimens with positive LN and 36 specimens with negative LN. Microarray hybridization on miRNA 4.0 chips (Affymetrix) was conducted at the genomic platform of the Institut Cochin, Paris.

本研究旨在评估1~2级或3级子宫内膜样腺癌的微小RNA（microRNA）表达谱是否与淋巴结状态相关，能否作为早期子宫内膜癌（EC，Endometrial Carcinoma）手术分期调整的辅助工具。研究对12例淋巴结阳性的福尔马林固定石蜡包埋（FFPE）子宫内膜癌原发肿瘤，以及36例按分级、分期与淋巴血管间隙浸润状态匹配的淋巴结阴性FFPE子宫内膜癌原发肿瘤，开展了微小RNA表达检测。总RNA提取严格遵循制造商说明书，采用miRNeasy FFPE试剂盒（Qiagen，法国库塔伯夫）完成。36例淋巴结阴性标本按匹配标准每3例合并为1份样本。本研究共对48份独立标本进行微阵列（microarray）分析，其中包括12份淋巴结阳性标本与36份淋巴结阴性标本。在巴黎科钦研究所的基因组平台上，完成了miRNA 4.0芯片（Affymetrix）的微阵列杂交实验。