Transcriptomic analysis of short-term 17a-ethynylestradiol exposure in two Californian sentinel fish species sardine (Sardinops sagax) and mackerel (Scomber japonicus)

In this study, we carried out RNA sequencing (RNAseq) of liver RNA harvested from wild sardine and mackerel exposed under laboratory conditions to EE2 and developed an analytical framework for transcriptomic analyses of species with limited genomic information. EE2 exposure altered expression patterns of key genes involved in important metabolic and physiological processes. The systems approach presented here provides a powerful tool for obtaining a comprehensive picture of endocrine disruption in aquatic organisms. Overall design: Examination of transcriptome changes in sentinel species exposed to a common, environmental compound (EE2).

本研究对实验室条件下暴露于乙炔雌二醇（EE2）的野生沙丁鱼与鲭鱼的肝脏RNA开展了RNA测序（RNAseq），并针对基因组信息有限的物种构建了转录组分析框架。EE2暴露可改变参与重要代谢与生理过程的关键基因的表达模式。本研究提出的系统方法可为全面解析水生生物的内分泌干扰效应提供强有力的研究工具。整体实验设计：对暴露于常见环境化合物（EE2）的指示物种的转录组变化进行检测分析。