Comparing genome-wide H3K36me3 levels in WT vs. dKDM4A mutant. Drosophila melanogaster

dKDM4A is a Drosophila H3 K9/K36 me2/me3 demethylase, which has been disrupted here by P-element insertion. We compare whole-genome ChIP-chip in mutant and WT to look for target genes of dKDM4A. Overall design: Two samples, WT and dkDM4A mutant. 2 biological replicates each. 2 arrays.

dKDM4A是果蝇（Drosophila）的组蛋白H3 K9/K36二甲基化/三甲基化去甲基化酶，本研究通过P因子插入突变使其功能失活。我们对突变体与野生型（Wild Type，缩写WT）样本开展全基因组ChIP-chip（染色质免疫沉淀芯片）分析，并对两组结果进行比较，以鉴定dKDM4A的靶基因。实验整体设计：共设置2组样本，即野生型与dKDM4A突变体；每组均设置2次生物学重复，共使用2张芯片。