Feedforward regulation of Myc coordinates lineage-specific with housekeeping gene expression during progenitor cell differentiation

Chromatin-associated RNA-Seq of pre-B cell line B3 transduced with Ikaros (Ikaros-ERt2) or Myc (Myc-ERt2) or Ikaros+Myc or control vector (Empty). Chromatin associated RNA from 4 or 2 biological replicates of B3 cells transduced with Ikaros, Myc, Ikaros+Myc were isolated 30, 60 and 180 min after induction with 4-OHT. Vector transduced B3 cells (ERt2-IRES-GFP) treated with 4-hydroxytamoxifen were used as controls.

本数据集针对转导了Ikaros（Ikaros-ERt2）、Myc（Myc-ERt2）、Ikaros与Myc联合转导或空载体（Empty）的前B细胞系（pre-B cell line）B3开展染色质相关RNA测序（Chromatin-associated RNA-Seq）。其中，转导Ikaros的B3细胞设置4个生物学重复，转导Myc、Ikaros+Myc的B3细胞各设置2个生物学重复；所有实验组细胞均在经4-羟基他莫昔芬（4-OHT）诱导30 min、60 min及180 min后提取染色质结合RNA。以转导ERt2-IRES-GFP载体并经4-羟基他莫昔芬（4-hydroxytamoxifen）处理的B3细胞作为对照。