Data_Sheet_1_Bioactive polyketides and meroterpenoids from the mangrove-derived fungus Talaromyces flavus TGGP35.PDF

Six new polyketides, which includes three new lactones (talarotones A–C) (1–3), one new polyketide (talarotide A) (4), two new polyenes (talaroyenes A, B) (5, 6), together with one new meroterpenoid (talaropenoid A) (7) and 13 known compounds (8–20) were isolated from the mangrove-derived fungus Talaromyces flavus TGGP35. The structure and configuration of the compounds 1–7 were elucidated from the data obtained from HR-ESI-MS, IR, 1D/2D NMR spectroscopy, Mo2 (OAc)4-induced electronic circular dichroism (ECD), CD spectroscopy, and modified Mosher's method. Compounds 5 and 20 displayed antioxidant activity with IC50 values of 0.40 and 1.36 mM, respectively. Compounds 3, 6, 11, 16, and 17 displayed cytotoxic activity against human cancer cells Hela, A549, and had IC50 values ranging from 28.89 to 62.23 μM. Compounds 7, 10–12, and 14–18 exhibited moderate or potent anti-insect activity against newly hatched larvae of Helicoverpa armigera Hubner, with IC50 values in the range 50–200 μg/mL. Compound 18 showed antibacterial activity against Ralstonia solanacearum with the MIC value of 50 μg/mL.

从红树林来源的黄篮状菌（*Talaromyces flavus*）TGGP35菌株中，分离得到7个全新化合物，其中包含6个聚酮类新化合物（polyketides）：3个新型内酯类化合物（talarotones A–C，编号1–3）、1个新型聚酮类化合物（talarotide A，编号4）、2个新型多烯类化合物（talaroyenes A、B，编号5、6），以及1个新型杂萜类化合物（meroterpenoid，talaropenoid A，编号7）；另获得13个已知化合物（编号8–20）。研究人员通过高分辨电喷雾电离质谱（HR-ESI-MS）、红外光谱（IR）、一维/二维核磁共振波谱（1D/2D NMR）、乙酸钼(II)诱导电子圆二色谱（Mo₂(OAc)₄-induced electronic circular dichroism, ECD）、圆二色谱（CD）光谱及改良莫斯杰法（modified Mosher's method），解析了化合物1–7的结构与绝对构型。活性评价结果显示：化合物5与20具有抗氧化活性，半数抑制浓度（IC₅₀）分别为0.40 mM和1.36 mM；化合物3、6、11、16和17对人宫颈癌细胞（Hela）、人肺癌细胞（A549）表现出细胞毒活性，IC₅₀值介于28.89~62.23 μM之间；化合物7、10–12及14–18对棉铃虫（*Helicoverpa armigera Hubner*）初孵幼虫具有中等至强效的杀虫活性，IC₅₀值范围为50~200 μg/mL；化合物18对青枯劳尔氏菌（*Ralstonia solanacearum*）具有抗菌活性，最低抑菌浓度（MIC）为50 μg/mL。