De novo transcriptome assembly of the green alga Ankistrodesmus falcatus

We aimed to create a de novo reference transcriptome of A. falcatus grown in batch culture. This assembly is valuable for researchers who need to parse apart genes that belong to zooplankton versus their algal food resource. A. falcatus is a member of many freshwater communities, so the reference transcriptome will also benefit metatranscriptomic analyses of freshwater phytoplankton diversity. Additionally, this assembly will be useful as a reference transcriptome for any other studies that require such molecular ecology resources.

本研究旨在构建批量培养条件下生长的A. falcatus的从头组装参考转录组（de novo reference transcriptome）。该组装结果可帮助研究者精准区分隶属于浮游动物（zooplankton）与其藻类食物源的基因，具备较高的应用价值。A. falcatus是众多淡水群落的组成类群，本参考转录组同样可助力淡水浮游植物（phytoplankton）多样性的宏转录组学（metatranscriptomic）分析。此外，该转录组组装结果还可作为参考转录组，为所有需要此类分子生态学研究资源的相关研究提供支撑。